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Center for Onchocerciasis Diagnostics 

The Center for Onchocerciasis Diagnostics at the University of South Florida College of Public Health is a research laboratory dedicated to the elimination of river blindness across Africa and Latin America. We are committed to supporting global health initiatives aimed at eradicating this disease.

River

Center for Onchocerciasis Diagnostics Focus

At the Center for Onchocerciasis Diagnostics, we are dedicated to conducting in-depth research on the epidemiology, diagnosis, and surveillance of onchocerciasis. Our team is committed to making significant contributions to the field of public health through our research efforts. Our goal is to provide valuable laboratory support and training to participating onchocerciasis elimination programs across the globe through collaborations with philanthropic organizations and other infectious disease support entities contributing to the global health efforts to protect against this debilitating disease known as river blindness.

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The Center's website provides access to laboratory manuals and SOPs for testing at risk populations for the level of exposure to Onchocerca volvulus using the Ov16 ELISA, as well as vector surveillance testing using the O150 PCR-ELISA. These laboratory manuals and Quality Control and Quality Assurance protocols are available to download in English, French, Portuguese, and Spanish for your convenience. Training documents and videos may be located under the training and educational materials tab.

 

We are an open access website dedicated to disseminating training materials and providing support to onchocerciasis elimination programs through the assistance of our collaborating partners (The Carter Center, The End Fund, Sightsavers & the Mectizan Donation Program).

Quick Links

External Quality Assurance & Laboratory Handbook - SOPs

Laboratory Methods for Onchocerciasis Elimination & External Quality Assurance

Please follow the "Read More" link below to access the onchocerciasis elimination manuals/handbooks:

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The External Quality Assurance Manual (USF-001)

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The Laboratory Handbook & SOPs 

(provides all the necessary information for the following protocols)

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Ov16 ELISA Protocol (OEPA Version) – Detection of antibodies against O. volvulus (USF-002)
DNA extraction from Simulium black flies to detect Onchocerca volvulus (USF-003)
Endpoint O-150 PCR Amplification of Onchocerca volvulus DNA (USF-004)
ELISA based detection of O-150 PCR products (USF-005)
E. coli ELISA Protocol – Detection of Antibodies for Sample Acceptance (USF-006)

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